KlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry
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KlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry
Many candidate unnatural DNA base pairs have been developed, but some of the best-replicated pairs adopt intercalated structures in free DNA that are difficult to reconcile with known mechanisms of polymerase recognition. Here we present crystal structures of KlenTaq DNA polymerase at different stages of replication for one such pair, dNaM-d5SICS, and show that efficient replication results fro...
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ژورنال
عنوان ژورنال: Nature Chemical Biology
سال: 2012
ISSN: 1552-4450,1552-4469
DOI: 10.1038/nchembio.966